Cellular Structures and Functions

Histology, the study of the microscopic structure of cells, tissues, and organs, uncovers how these entities maintain the vitality of organisms. At the cellular level, each cell functions as a fundamental unit of life, encapsulated by a plasma membrane which distinguishes its boundaries. Within, the cytoplasm houses various organelles, each with distinct roles, all suspended in the cytosol—the aqueous part of the cytoplasm.


The Nucleus: Considered the control center, it governs cellular activity by directing protein synthesis and contains most of the cell's genetic material in the form of DNA. Learn more about the cell nucleus and its function at Kenhub.


Mitochondria: Known as the powerhouse, mitochondria produce chemical energy in the form of ATP, which is vital for the survival of cells.


Ribosomes: These molecular machines are responsible for protein synthesis, reading RNA transcripts and assembling proteins, essential for cellular functions.


Endoplasmic Reticulum (ER): The ER has a twofold function, with a rough part studded with ribosomes for protein synthesis and a smooth part that synthesizes lipids and detoxifies certain chemicals.


Golgi Apparatus: This organelle modifies, sorts, and packages proteins and lipids for delivery to targeted destinations.


Lysosomes: They contain enzymes that break down and digest unneeded cellular components.


At a broader anatomical perspective, tissues emerge from a collective of cells specialized for a common function, which then integrates to form organs, each performing a specific task essential to an organism's health. Glycoproteins, for instance, are critical at both cellular and tissue levels, acting in cell adhesion and recognition, thereby contributing to the overall structural and functional cohesion within an organ system.


Understanding these components at the microscopic level illuminates the elegant complexity of biological systems and reinforces the central role of histology in the study of anatomy and physiology.


May 7, 2025
Our latest study addresses a critical challenge in liver tissue engineering: stem cell-derived liver cells (iHeps) typically remain functionally immature, limiting their usefulness for drug testing and disease modeling. Our research team created 3D microtissues using droplet microfluidics technology by: • Encapsulating iHeps in tiny collagen gel droplets (~250 μm diameter) • Coating these structures with various non-parenchymal cells (NPCs) • Testing different combinations and sequences of supporting cells Key findings: 1) Embryonic fibroblasts and liver sinusoidal endothelial cells (LSECs) produced the most mature iHeps compared to other tested cell types 2) Sequential application of cell signals (embryonic fibroblasts first, then LSECs) yielded optimal maturation 3) Specific growth factors like stromal-derived factor-1 alpha were identified as important maturation enhancers 4) Gene expression analysis confirmed that LSEC/iHep microtissues closely resembled adult human liver cells This platform enables researchers to identify critical cellular interactions and molecular signals that drive liver cell maturation, providing valuable insights for developing more physiologically relevant liver models for drug screening and regenerative medicine applications. https://www.sciencedirect.com/science/article/pii/S174270612500193X SIMPLE SUMMARY: Embryonic fibroblasts and liver sinusoidal endothelial cells dramatically improved iHep maturation compared to other cell types tested, producing more functionally mature liver cells. Sequential application proved crucial—adding embryonic fibroblasts first, followed by endothelial cells, yielded optimal maturation. Specific growth factors including stromal-derived factor-1 enhanced this process. This research enables creation of more authentic mini-liver tissues that function like human liver. These improved models support better drug testing, disease research, and regenerative medicine applications.
December 13, 2024
1. Three-Dimensional (3D) Cell Culture Techniques : New 3D cell culture methods have significantly improved the properties of stem cells, enhancing their viability and functionality for tissue regeneration. These techniques allow for more accurate modeling of tissue architecture and function. 2. Engineered Stem Cells : Advances in bioengineering have led to the development of "engineered stem cells," which are modified to enhance their regenerative capabilities. These next-generation stem cells are designed to be more effective in tissue repair and regeneration. 3. Injectable Biomimetic Hydrogels : Researchers have developed advanced injectable hydrogels that mimic natural tissue environments. These hydrogels hold significant promise for tissue engineering applications, providing a supportive matrix for stem cell growth and differentiation. 4. Integration with Tissue Scaffolds : There have been significant improvements in integrating stem cells with biomaterial scaffolds. These scaffolds provide structural support and enhance the differentiation and growth of stem cells into specific tissue types, improving the outcomes of regenerative treatments. 5. Gene Editing and mRNA Technologies : Techniques like CRISPR and mRNA-based therapies are being used to modify stem cells at the genetic level, enhancing their ability to regenerate tissues. These technologies allow for precise control over stem cell behavior and function.
December 13, 2024
The MTM lab has experienced considerable growth over the last several years at the University of Illinois Chicago!
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