Cellular Structures and Functions

Histology, the study of the microscopic structure of cells, tissues, and organs, uncovers how these entities maintain the vitality of organisms. At the cellular level, each cell functions as a fundamental unit of life, encapsulated by a plasma membrane which distinguishes its boundaries. Within, the cytoplasm houses various organelles, each with distinct roles, all suspended in the cytosol—the aqueous part of the cytoplasm.


The Nucleus: Considered the control center, it governs cellular activity by directing protein synthesis and contains most of the cell's genetic material in the form of DNA. Learn more about the cell nucleus and its function at Kenhub.


Mitochondria: Known as the powerhouse, mitochondria produce chemical energy in the form of ATP, which is vital for the survival of cells.


Ribosomes: These molecular machines are responsible for protein synthesis, reading RNA transcripts and assembling proteins, essential for cellular functions.


Endoplasmic Reticulum (ER): The ER has a twofold function, with a rough part studded with ribosomes for protein synthesis and a smooth part that synthesizes lipids and detoxifies certain chemicals.


Golgi Apparatus: This organelle modifies, sorts, and packages proteins and lipids for delivery to targeted destinations.


Lysosomes: They contain enzymes that break down and digest unneeded cellular components.


At a broader anatomical perspective, tissues emerge from a collective of cells specialized for a common function, which then integrates to form organs, each performing a specific task essential to an organism's health. Glycoproteins, for instance, are critical at both cellular and tissue levels, acting in cell adhesion and recognition, thereby contributing to the overall structural and functional cohesion within an organ system.


Understanding these components at the microscopic level illuminates the elegant complexity of biological systems and reinforces the central role of histology in the study of anatomy and physiology.


October 18, 2025
𝗦𝗶𝗺𝗽𝗹𝗲 𝗦𝘂𝗺𝗺𝗮𝗿𝘆: This study explores how we can improve lab-grown liver cells for medical research and drug testing. The MTMLab team works with induced pluripotent stem cells (iPSCs) - special cells that can be transformed into liver-like cells - because real human liver cells are hard to obtain. However, these lab-grown liver cells don't function as well as mature adult liver cells. The research team discovered that the surface environment where these cells grow is crucial for their development. We created tiny fiber scaffolds made from different materials like collagen, decellularized livers, and chitosan that mimic the natural structure around liver cells. When liver cells were grown on these specially designed nanofibers for three weeks, they displayed higher function compared to cells grown on standard surfaces. Our key finding was that both the material composition and the nanoscale fiber structure were important - stiffer synthetic fibers or softer materials without the appropriate topography or composition prevented proper cell maturation. This research helps create better lab models of human liver tissue that can be used for testing new drugs and studying liver diseases more effectively.
October 7, 2025
Owen Lally Modeling the synergistic effects of alcohol and fats on liver disease via engineered cocultures In Vitro Liver Toxicology Testing of Rat and Dog Hepatocytes to Reduce In Vivo Regulatory Requirements Nathan Shelton Enhancing the Functions and Hepatitis B Virus Infectability of Primary Human Hepatocytes Protein Microarrays to Probe Synergistic Effects of Extracellular Matrix Composition and Stiffness on Liver Macrophages Lesly Villarreal Engineering a 3D Placental Trophoblast Invasion Platform Via Droplet Microfluidics Gas-permeable Plates to Model Synergetic Effects of Oxygen and Endothelial Factors on Liver Zonation Emanuele Spanghero Modeling the Interplay Between Liver and Heart Diseases via a Human Dual-Organ Platform Engineering High Cell Density Beating Cords of Cardiomyocytes and Fibroblasts via Photopatterned Alginate
May 7, 2025
Our latest study addresses a critical challenge in liver tissue engineering: stem cell-derived liver cells (iHeps) typically remain functionally immature, limiting their usefulness for drug testing and disease modeling. Our research team created 3D microtissues using droplet microfluidics technology by: • Encapsulating iHeps in tiny collagen gel droplets (~250 μm diameter) • Coating these structures with various non-parenchymal cells (NPCs) • Testing different combinations and sequences of supporting cells Key findings: 1) Embryonic fibroblasts and liver sinusoidal endothelial cells (LSECs) produced the most mature iHeps compared to other tested cell types 2) Sequential application of cell signals (embryonic fibroblasts first, then LSECs) yielded optimal maturation 3) Specific growth factors like stromal-derived factor-1 alpha were identified as important maturation enhancers 4) Gene expression analysis confirmed that LSEC/iHep microtissues closely resembled adult human liver cells This platform enables researchers to identify critical cellular interactions and molecular signals that drive liver cell maturation, providing valuable insights for developing more physiologically relevant liver models for drug screening and regenerative medicine applications. https://www.sciencedirect.com/science/article/pii/S174270612500193X SIMPLE SUMMARY: Embryonic fibroblasts and liver sinusoidal endothelial cells dramatically improved iHep maturation compared to other cell types tested, producing more functionally mature liver cells. Sequential application proved crucial—adding embryonic fibroblasts first, followed by endothelial cells, yielded optimal maturation. Specific growth factors including stromal-derived factor-1 enhanced this process. This research enables creation of more authentic mini-liver tissues that function like human liver. These improved models support better drug testing, disease research, and regenerative medicine applications.